December 9, 2015
A new study by the group of Professor Sebastian Springer at Jacobs University helps explain how viruses manage to go undetected when they infect body cells. They have shown that the gp40 protein of the murine cytomegalovirus (mCMV) binds to cellular proteins that are essential for the antiviral immune defense and holds them back inside the cell. This blocks the immune response against mCMV.
Major histocompatibility complex (MHC) class I molecules play a pivotal role in the immune defense against intracellular parasites, such as viruses. Inside the cell, they selectively bind small pieces of the virus that are generated during infection and transport them to the cell surface to display the infection to the immune system, a process called antigen presentation. Specialized immune cells, the cytotoxic T lymphocytes (CTL), can recognize those viral pieces and, in turn, kill the infected cell in order to fight the infection (see left part of figure).
It is not astonishing that many viruses aim to interfere with antigen presentation by MHC class I molecules to circumvent elimination. Herpesviruses are masters of immunoevasion and possess a series of multiple interfering proteins, the immunoevasins: The gp40 protein of mCMV inhibits the transport of MHC class I molecules to the cell surface and thus viral recognition by CTL. Instead, MHC class I molecules are retained inside the cell (see right part of figure)
"We have shown for the first time that gp40 binds to MHC class I molecules", says Professor Springer. "We do not yet know how gp40 itself is kept inside the cell, but we believe that it uses another protein as some sort of anchor." The researchers identified a region in the gp40 protein, the linker, which probably binds to this unknown cellular retention factor (see right part of figure).
"It is an amazingly effective strategy for a virus to escape from the immune response", concludes Professor Springer. "Cytomegaloviruses and other herpesviruses infect humans and animals and cause many diseases. We need to understand more about immune escape so that effective treatments can be designed."
The findings will soon be published in the “Journal of Cell Science”: Publication Linda Janßen, Venkat Raman Ramnarayan, Mohamed Aboelmagd, Maria Iliopoulou, Zeynep Hein, Irina Majoul, Susanne Fritzsche, Anne Halenius, and Sebastian Springer: “The murine cytomegalovirus immunoevasin gp40 binds MHC class I molecules to retain them in the early secretory pathway”, Journal of Cell Science, 2015. The study was financed in part by the Tönjes Vagt Foundation of Bremen.
Contact Sebastian Springer for further information:
s.springer [at] jacobs-university.de | Tel.: +49 421 200- 3243